{"id":10971,"date":"2024-02-28T11:05:00","date_gmt":"2024-02-28T10:05:00","guid":{"rendered":"https:\/\/bio-pat.org\/?p=10971"},"modified":"2024-07-09T08:06:17","modified_gmt":"2024-07-09T07:06:17","slug":"collaboration-elosystems-and-kiwi-biolab","status":"publish","type":"post","link":"https:\/\/bio-pat.org\/en\/collaboration-elosystems-and-kiwi-biolab","title":{"rendered":"Collaboration EloSystems and KIWI-biolab, TU Berlin"},"content":{"rendered":"\n

Collaboration EloSystems and KIWI-biolab, TU Berlin<\/strong><\/p>\n\n\n\n

In the frame of a PhD project of Lucas Kaspersetz at the KIWI-biolab<\/a>, Chair of Bioprocess Engineering at TU Berlin, the new instrument of EloSystems<\/a> – “\u00b5Check<\/a>” – a smart system for online monitoring of bacteria in bioprocesses was tested in E. coli <\/em>cultivations. <\/p>\n\n\n\n

These E. coli<\/em> fed-batch cultivations were conducted in a robotic small scale bioreactor system with 8 parallel running cultivation vessels of 100 mL fermentation volume (Cultivation system HEL BioXplorer 100). The cultivation system was integrated into a liquid handling station (LHS Tecan Evo 150) for automated cultivation workflows. The aim of these parallel cultivations was to investigate the responses of E. coli<\/em> related to recombinant protein production under glucose oscillations and temperature shifts.<\/p>\n\n\n\n

The \u00b5Check \u201cclassic\u201d device was used in combination with the bypass module, functioning as a gas bubble separator, for the online measurement of OD, cell size (length), and cell concentration in these E. coli<\/em> cultivations. With a special optical flow cell, processes up to OD 60 can be tracked online without dilution or sampling. In these cultivations, OD values of up to 45 were obtained. At OD > 60, a dilution option is available allowing measurements in high cell density cultivations of up to OD 200 in automatic operation.<\/p>\n\n\n\n

Results <\/strong>of the online measurements with \u00b5Check in E. coli <\/em>parallel cultivations:
– Good correlation was achieved between the biomass and reference measurements (at-line).
– Significant changes in cell morphology due to exposure to stress were observed with the \u00b5Check device.<\/p>\n\n\n\n

Conclusions<\/strong> of the feasibility study for the new measurement device:
– Wide concentration ranges can be covered without sample loss due to the the design of the narrow flow cell -> suitable for aerobic, heavily gassed systems.
– The residence volume in the bubble separator will be further improved to allow the use in a small scale with parallel-operated cultivation vessels from approx. 10-20 mL sample volume. <\/p>\n\n\n\n